Was coronavirus really in Europe in March 2019?


Claire Crossan, Glasgow Caledonian University

The novel coronavirus – SARS-CoV-2 – may have been in Europe for longer than previously thought. Recent studies have suggested that it was circulating in Italy as early as December 2019. More surprisingly, researchers at the University of Barcelona found traces of the virus when testing untreated wastewater samples dated March 12, 2019.

The study was recently published on a preprint server, medRxiv. The paper is currently being subject to critical review by outside experts in preparation for publication in a scientific journal. Until this process of peer review has been completed, though, the evidence needs to be treated with caution.

So, how was the experiment conducted and what exactly did the scientists find?

One of the early findings about SARS-CoV-2 is that it is found in the faeces of infected people. As the virus makes its way through the gut – where it can cause gastrointestinal symptoms – it loses its outer protein layer, but bits of genetic material called RNA survive the journey intact and are “shed” in faeces. At this point, it is no longer infectious – as far as current evidence tells us.

But the fact that these bits of coronavirus RNA can be found in untreated wastewater (known as “influent”) is useful for tracking outbreaks. Indeed, they can predict where an outbreak is likely to occur a week to ten days before they show up in official figures – the reason being that people shed coronavirus before symptoms become evident. These “pre-symptomatic” people then have to get sick enough to be tested, get the results, and be admitted to a hospital as an official “case”, hence the week or so lag.

As a result, many countries, including Spain, are now monitoring wastewater for traces of coronavirus. In this particular study, wastewater epidemiologists were examining frozen samples of influent between January 2018 and December 2019 to see when the virus made its debut in the city.

Experts around the world are monitoring wastewater for signs of coronavirus.
arhendrix/Shutterstock.com

They found evidence of the virus on January 15, 2020, 41 days before the first official case was declared on February 25, 2020. All the samples before this date were negative, except for a sample from March 12, 2019, which gave a positive result in their PCR test for coronavirus. PCR is the standard way of testing to see if someone currently has the disease.

PCR involves getting samples of saliva, mucus, frozen wastewater or whatever else the virus is thought to be lurking in, clearing all the unnecessary stuff out of the sample, then converting the RNA – which is a single strand of genetic material – into DNA (the famous double-stranded helix). The DNA is then “amplified” in successive cycles until key bits of genetic material that are known to only exist in a particular virus are plentiful enough to be detected with a fluorescent probe.

Not highly specific

In coronavirus testing, scientists typically screen for more than one gene. In this case, the researchers tested for three. They had a positive result for the March 2019 sample in one of the three genes tested – the RdRp gene. They screened for two regions of this gene and both were only detected around the 39th cycle of amplification. (PCR tests become less “specific” with increasing rounds of amplification. Scientists generally use 40 to 45 rounds of amplification.)

There are several explanations for this positive result. One is that SARS-CoV-2 is present in the sewage at a very low level. Another is that the test reaction was accidentally contaminated with SARS-CoV-2 in the laboratory. This sometimes happens in labs as positive samples are regularly being handled, and it can be difficult to prevent very small traces of positive sample contaminating others.

Another explanation is that there is other RNA or DNA in the sample that resembles the test target site enough for it to give a positive result at the 39th cycle of amplification.

Further tests need to be carried out to conclude that the sample contains SARS-CoV-2, and a finding of that magnitude would need to be replicated separately by independent laboratories.

Reasons to be circumspect

A curious thing about this finding is that it disagrees with epidemiological data about the virus. The authors don’t cite reports of a spike in the number of respiratory disease cases in the local population following the date of the sampling.

Also, we know SARS-CoV-2 to be highly transmissible, at least in its current form. If this result is a true positive it suggests the virus was present in the population at a high enough incidence to be detected in an 800ml sample of sewage, but then not present at a high enough incidence to be detected for nine months, when no control measures were in place.

So, until further studies are carried out, it is best not to draw definitive conclusions.The Conversation

Claire Crossan, Research Fellow, Virology, Glasgow Caledonian University

This article is republished from The Conversation under a Creative Commons license. Read the original article.

Mali: Islamists on the March with Child Soldiers


The link below is to an article reporting on the use of child soldiers by Islamists in northern Mali.

For more visit:
http://www.chron.com/news/nation-world/article/Islamists-in-Mali-recruit-pay-for-child-soldiers-3917360.php

Article: Noah – the Movie


The following link is to an article reporting on the upcoming movie about Noah, called ‘Noah.’ the film is set to star Russell Crowe as Noah. The film is set for release in the USA during March 2014.

For more, visit:
http://www.christiantelegraph.com/issue16135.html

Japan: Tsunami Debris Heading for USA


The following article is an interesting report on debris from the Japanese Tsunami event of March 2011.

For more, visit:
http://www.foxnews.com/scitech/2012/02/15/25-million-tons-tsunami-debris-floating-toward-us-shores/

17 more Christian men thrown into prison in Eritrea


Military officials on Saturday, March 27, 2010, arrested 17 young men gathered for prayer in a town called Segenaite in southern Eritrea, Africa. The men are apparently Christian soldiers doing their compulsory national military service. They belong to various churches, reports Open Doors USA.

The men are being held in a Segenaite Police Station prison cell. It is not clear whether they will be moved to another of Eritrea’s detention centers.

These arrests bring to 28 the reported number of Christians arrested since the beginning of March for their refusal to stop worshiping outside of the government sanctioned Eritrean Orthodox, Catholic and Evangelical Lutheran churches.

Sources announced in February that approximately 2,200 Christians remain in prison for their refusal to stop practicing their faith outside of the government sanctioned religious groups. The figure is significantly lower than the figure released at the beginning of 2009. Sources explained to Open Doors that many of those released were let go because of poor health. Most were also released on very strict bail conditions. Additionally, many other Christians have been released only to be sent back to the military in anticipation of a renewed war with neighboring Ethiopia. At least 12 Christians have died while being incarcerated in Eritrean prison camps.

Eritrea is ranked No. 11 on this year’s Open Doors World Watch List of 50 countries which are the worst persecutors of Christians.

Open Doors recently received the translation of a letter from a pastor of an Eritrean church written to his wife from prison.

My dearest wife;

God, by His holy will, has prolonged my prison sentence to five years and four month. I very much long for the day that I will be reunited with you my dear wife, our children and God’s people in the church.

My dear, listen to me; not only as a wife, but also as a Christian woman who has come to understand who God is and how deep and mysterious His ways are. Yes! I love you, I love the children and I would love to be free in order to serve God. But, in here, God has made me not only a sufferer for His Name’s sake in a prison of this world over which Christ has won victory, but also a prisoner of His indescribable love and grace.

I am testing and experiencing the love and care of our Lord every day. When they first brought me to this prison, I had thoughts which were contrary to what the Bible says. I thought the devil had prevailed over the church and over me. I thought the work of the gospel in Eritrea was over. But it did not take one day for the Lord to show me that He is a sovereign God and that He is in control of all things – even here in prison.

The moment I entered my cell, one of the prisoners called me and said, ‘Pastor, come over here. Everyone in this cell is unsaved. You are very much needed here.’ So, on the same day I was put in prison, I carried on my spiritual work.

My dear, the longer I stay in here, the more I love my Savior and tell the people here about His goodness. His grace is enabling me to overcome the coldness and the longing that I feel for you and for our children. Sometimes I ask myself, ‘Am I out of my mind? Am I a fool?’ Well, isn’t that what the apostle had said, ‘Whether I am of sound mind or out of my mind, it is for the sake of Christ.’ (2 Cor. 5:13)

My most respected wife, I love you more than I can say. Please help the children understand that I am here as a prisoner of Christ for the greater cause of the gospel.

— From a pastor in bonds in Eritrea

An estimated 100 million Christians worldwide suffer interrogation, arrest and even death for their faith in Christ, with millions more facing discrimination and alienation. Open Doors supports and strengthens believers in the world’s most difficult areas through Bible and Christian literature distribution, leadership training and assistance, Christian community development, prayer and presence ministry and advocacy on behalf of suffering believers.

Report from the Christian Telegraph 

International Christian Concern: Persecution News from China and Indonesia – March 2010